Mouse fetal intestinal organoids: new model to study epithelial maturation from suckling to weaning.

ABSTRACT: During the suckling-to-weaning transition, the intestinal epithelium matures, allowing digestion of solid food. Transplantation experiments with rodent fetal epithelium into subcutaneous tissue of adult animals suggest that this transition is intrinsically programmed and occurs in the absence of dietary or hormonal signals. Here, we show that organoids derived from mouse primary fetal intestinal epithelial cells express markers of late fetal and neonatal development. In a stable culture medium, these fetal epithelium-derived organoids lose all markers of neonatal epithelium and start expressing hallmarks of adult epithelium in a time frame that mirrors epithelial maturation in vivoIn vitro postnatal development of the fetal-derived organoids accelerates by dexamethasone, a dr

SUBMITTER: Navis M 

PROVIDER: S-EPMC6362357 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature