Siberian larch (Larix sibirica Ledeb.) chloroplast genome and development of polymorphic chloroplast markers.

ABSTRACT:

Background

The main objectives of this study were sequencing, assembling, and annotation of chloroplast genome of one of the main Siberian boreal forest tree conifer species Siberian larch (Larix sibirica Ledeb.) and detection of polymorphic genetic markers - microsatellite loci or simple sequence repeats (SSRs) and single nucleotide polymorphisms (SNPs).

Results

We used the data of the whole genome sequencing of three Siberian larch trees from different regions - the Urals, Krasnoyarsk, and Khakassia, respectively. Sequence reads were obtained using the Illumina HiSeq2000 in the Laboratory of Forest Genomics at the Genome Research and Education Center of the Siberian Federal University. The assembling was done using the Bowtie2 mapping program and the SPAdes genomic assembl

SUBMITTER: Bondar EI 

PROVIDER: S-EPMC6362560 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature