Manipulation of Nephron-Patterning Signals Enables Selective Induction of Podocytes from Human Pluripotent Stem Cells.

ABSTRACT:

Background

Previous research has elucidated the signals required to induce nephron progenitor cells (NPCs) from pluripotent stem cells (PSCs), enabling the generation of kidney organoids. However, selectively controlling differentiation of NPCs to podocytes has been a challenge.

Methods

We investigated the effects of various growth factors in cultured mouse embryonic NPCs during three distinct steps of nephron patterning: from NPC to pretubular aggregate, from the latter to epithelial renal vesicle (RV), and from RV to podocyte. We then applied the findings to human PSC-derived NPCs to establish a method for selective induction of human podocytes.

Results

Mouse NPC differentiation experiments revealed that phase-specific manipulation of Wnt and Tgf-β signaling

SUBMITTER: Yoshimura Y 

PROVIDER: S-EPMC6362617 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature