Dppa2 and Dppa4 directly regulate the Dux-driven zygotic transcriptional program.

ABSTRACT: The molecular regulation of zygotic genome activation (ZGA) in mammals remains an exciting area of research. Primed mouse embryonic stem cells contain a rare subset of "2C-like" cells that are epigenetically and transcriptionally similar to the two-cell embryo and thus represent an in vitro approximation for studying ZGA transcription regulation. Recently, the transcription factor Dux, expressed in the minor wave of ZGA, was described to activate many downstream ZGA transcripts. However, it remains unknown what upstream maternal factors initiate ZGA in either a Dux-dependent or Dux-independent manner. Here we performed a candidate-based overexpression screen, identifying, among others, developmental pluripotency-associated 2 (Dppa2) and Dppa4 as positive regulators of 2C-like cells and tra

SUBMITTER: Eckersley-Maslin M 

PROVIDER: S-EPMC6362816 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature