MiR-135a suppresses migration of gastric cancer cells by targeting TRAF5-mediated NF-κB activation.

ABSTRACT:

Background

As crucial regulators and possible biomarkers for cancer development, miRNAs have attracted intensive attention during the last two decades. Among the known miRNAs, miR-135a has been indicated as a tumor suppressor in several cancer types, whereas its roles and mechanisms in gastric cancer (GC) remain largely unclear.

Materials and methods

Quantitative PCR (qPCR) was conducted to detect the expression of miR-135a in paired GC tissues as well as cell lines. The prognostic value was evaluated by Kaplan-Meier survival analysis. Wound healing and transwell assays were performed to determine the roles of miR-135a in GC cell migration. Dual-luciferase reporter assay, qPCR, and Western blot analysis were used to validate the targeting of TRAF5 and subsequent NF-κB pathwa

SUBMITTER: Xie Y 

PROVIDER: S-EPMC6362934 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature