Ratiometric Fluorescence Coding for Multiplex Nucleic Acid Amplification Testing.

ABSTRACT: Although nucleic acid amplification testing (NAAT) has become the cornerstone for molecular diagnosis of diseases, expanding the multiplexed detection capacity of NAAT remains an important objective. To this end, encoding each nucleic acid target with a specific fluorescently labeled probe has been the most mature approach for multiplexed detection. Unfortunately, the number of targets that can be differentiated via this one-target-one-fluorophore multiplexed detection approach is restricted by spectral overlaps between fluorophores. In response, we present herein a new multiplexed detection approach termed ratiometric fluorescence coding, in which we encode each nucleic acid target with a specific ratio between two standard fluorophores. In ratiometric fluorescence coding, we employ the p

SUBMITTER: Zhang Y 

PROVIDER: S-EPMC6363117 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature