Discovery of Leptospira spp. seroreactive peptides using ORFeome phage display.

ABSTRACT:

Background

Leptospirosis is the most common zoonotic disease worldwide. The diagnostic performance of a serological test for human leptospirosis is mainly influenced by the antigen used in the test assay. An ideal serological test should cover all serovars of pathogenic leptospires with high sensitivity and specificity and use reagents that are relatively inexpensive to produce and can be used in tropical climates. Peptide-based tests fulfil at least the latter two requirements, and ORFeome phage display has been successfully used to identify immunogenic peptides from other pathogens.

Methodology/principal findings

Two ORFeome phage display libraries of the entire Leptospira spp. genomes from five local strains isolated in Malaysia and seven WHO reference strains were constr

SUBMITTER: Ramli SR 

PROVIDER: S-EPMC6363232 | biostudies-literature | 2019 Jan

REPOSITORIES: biostudies-literature