The Rab27a effectors JFC1/Slp1 and Munc13-4 regulate exocytosis of neutrophil granules.

ABSTRACT: Neutrophil granules contain secretory molecules that contribute to the implementation of all neutrophil functions. The molecular components that regulate the exocytosis of neutrophil granules have not been characterized. In this study, using small interfering RNA gene-targeting approaches and granulocytes from genetically modified mice, we characterized the Rab27a effectors JFC1/Slp1 and Munc13-4 as components of the exocytic machinery of granulocytes. Using total internal reflection fluorescence microscopy analysis, we show that Rab27a and JFC1 colocalize in predocked and docked vesicles in granulocytes. Next, we demonstrate that JFC1-downregulated granulocytes have impaired myeloperoxidase secretion. Using immunological interference, we confirm that JFC1 plays an important role in azurop

SUBMITTER: Brzezinska AA 

PROVIDER: S-EPMC6363534 | biostudies-literature | 2008 Dec

REPOSITORIES: biostudies-literature