Oxidative stress induces release of 2'-AMP from microglia.

ABSTRACT:

Background

Microglia metabolize exogenous 2'-AMP and 3'-AMP (non-canonical nucleotides) to adenosine and exogenous 2'-AMP and 3'-AMP (via conversion to adenosine) inhibit the production of inflammatory cytokines by microglia. This suggests that if microglia release endogenous 2'-AMP and/or 3'-AMP in response to injurious stimuli, this would complete an autocrine/paracrine mechanism that attenuates the over-activation of microglia during brain injury. Here we investigated in microglia (and for comparison astrocytes and neurons) the effects of injurious stimuli on extracellular and intracellular levels of 2',3'-cAMP (2'-AMP and 3'-AMP precursor), 2'-AMP, and 3'-AMP.

Methods

Experiments were conducted in primary cultures of rat microglia, astrocytes, and neurons. Cells were exp

SUBMITTER: Jackson TC 

PROVIDER: S-EPMC6363863 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature