N-terminal phosphorylation of xHes1 controls inhibition of primary neurogenesis in Xenopus.

ABSTRACT: The processes of cell proliferation and differentiation are intimately linked during embryogenesis, and the superfamily of (basic) Helix-Loop-Helix (bHLH) transcription factors play critical roles in these events. For example, neuronal differentiation is promoted by class II bHLH proneural proteins such as Ngn2 and Ascl1, while class VI Hes proteins act to restrain differentiation and promote progenitor maintenance. We have previously described multi-site phosphorylation as a key regulator of tissue specific class II bHLH proteins in all three embryonic germ layers, and this enables coordination of differentiation with the cell cycle. Hes1 homologues also show analogous conserved proline directed kinase sites. Here we have used formation of Xenopus primary neurons to investigate the effect

SUBMITTER: Hardwick LJA 

PROVIDER: S-EPMC6363968 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature