Cell Cycle Arrest in G₂/M Phase Enhances Replication of Interferon-Sensitive Cytoplasmic RNA Viruses via Inhibition of Antiviral Gene Expression.

ABSTRACT: Vesicular stomatitis virus (VSV) (a rhabdovirus) and its variant VSV-ΔM51 are widely used model systems to study mechanisms of virus-host interactions. Here, we investigated how the cell cycle affects replication of these viruses using an array of cell lines with different levels of impairment of antiviral signaling and a panel of chemical compounds arresting the cell cycle at different phases. We observed that all compounds inducing cell cycle arrest in G₂/M phase strongly enhanced the replication of VSV-ΔM51 in cells with functional antiviral signaling. G₂/M arrest strongly inhibited type I and type III interferon (IFN) production as well as expression of IFN-stimulated genes in response to exogenously added IFN. Moreover, G₂/M arrest enhanced the replica

SUBMITTER: Bressy C 

PROVIDER: S-EPMC6364032 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature