Peptide-siRNA Supramolecular Particles for Neural Cell Transfection.

ABSTRACT: Small interfering ribonucleic acid (siRNA)-based gene knockdown is an effective tool for gene screening and therapeutics. However, the use of nonviral methods has remained an enormous challenge in neural cells. A strategy is reported to design artificial noncationic modular peptides with amplified affinity for siRNA via supramolecular assembly that shows efficient protein knockdown in neural cells. By solid phase synthesis, a sequence that binds specifically double-stranded ribonucleic acid (dsRNA) with a self-assembling peptide for particle formation is integrated. These supramolecular particles can be further functionalized with bioactive sequences without affecting their biophysical properties. The peptide carrier is found to silence efficiently up to 83% of protein expression in primar

SUBMITTER: Hernandez-Garcia A 

PROVIDER: S-EPMC6364495 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature