Kinetic insights into the role of the reductant in H₂O₂-driven degradation of chitin by a bacterial lytic polysaccharide monooxygenase.

ABSTRACT: Lytic polysaccharide monooxygenases (LPMOs) are monocopper enzymes that catalyze oxidative cleavage of glycosidic bonds in polysaccharides in the presence of an external electron donor (reductant). In the classical O₂-driven monooxygenase reaction, the reductant is needed in stoichiometric amounts. In a recently discovered, more efficient H₂O₂-driven reaction, the reductant would be needed only for the initial reduction (priming) of the LPMO to its catalytically active Cu(I) form. However, the influence of the reductant on reducing the LPMO or on H₂O₂ production in the reaction remains undefined. Here, we conducted a detailed kinetic characterization to investigate how the reductant affects H₂O₂-driven degradation

SUBMITTER: Kuusk S 

PROVIDER: S-EPMC6364757 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature