Mesenchymal stem cells with downregulated Hippo signaling attenuate lung injury in mice with lipopolysaccharide‑induced acute respiratory distress syndrome.

ABSTRACT: Mesenchymal stem cell (MSC)‑mediated repair of injured alveolar epithelial cells is a promising potential cure for acute respiratory distress syndrome (ARDS); however, the repairing effect of MSCs is limited by poor homing and differentiation. Our previous study revealed that the inhibition of the Hippo signaling pathway promotes the proliferation, migration and differentiation of MSCs in vitro, leading to the hypothesis that MSCs with downregulated Hippo signaling could further ameliorate lipopolysaccharide (LPS)‑induced ARDS in vivo. In the current study, mouse bone marrow‑derived MSCs (mMSCs) with downregulated Hippo signaling were constructed by shRNA‑mediated knockdown of large tumor suppressor kinase 1 (Lats1) and were intratracheally administered to LPS‑induced mouse models of ARDS.

SUBMITTER: Li L 

PROVIDER: S-EPMC6365074 | biostudies-literature | 2019 Mar

REPOSITORIES: biostudies-literature