A Multi-model Approach to Assessing Local and Global Cryo-EM Map Quality.

ABSTRACT: There does not currently exist a standardized indicator of how well cryo-EM-derived models represent the density from which they were generated. We present a straightforward methodology that utilizes freely available tools to generate a suite of independent models and to evaluate their convergence in an EM density. These analyses provide both a quantitative and qualitative assessment of the precision of the models and their representation of the density, respectively, while concurrently providing a platform for assessing both global and local EM map quality. We further use standardized datasets to provide an expected deviation within a suite of models refined against EM maps reported to be at 5 Å resolution or better. Associating multiple atomic models with a deposited EM map provides a ra

SUBMITTER: Herzik MA 

PROVIDER: S-EPMC6365196 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature