β-Actin: Not a Suitable Internal Control of Hepatic Fibrosis Caused by Schistosoma japonicum.

ABSTRACT: Schistosomiasis japonica is a significant health problem that leads to morbidity and mortality of humans. It is characterized by hepatic granulomatous response and fibrosis caused by eggs deposition in the liver. β-actin, a traditional housekeeping gene, is widely used as an internal control to normalize gene and protein expression. However, β-actin expression can fluctuate upon the treatment with pharmacological agents or under some physiological and pathological conditions. In this study, we found that the expressions of both β-actin mRNA and protein increased significantly with hepatic fibrosis formation after 6 weeks infection with Schistosoma japonicum and kept high level during the progression of hepatic fibrosis, while the levels of β-Tubulin and glyceraldehyde-3-phosphate de

SUBMITTER: Zhang B 

PROVIDER: S-EPMC6365423 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature