Predictable Molecular Adaptation of Coevolving Enterococcus faecium and Lytic Phage EfV12-phi1.

ABSTRACT: Bacteriophages are highly abundant in human microbiota where they coevolve with resident bacteria. Phage predation can drive the evolution of bacterial resistance, which can then drive reciprocal evolution in the phage to overcome that resistance. Such coevolutionary dynamics have not been extensively studied in human gut bacteria, and are of particular interest for both understanding and eventually manipulating the human gut microbiome. We performed experimental evolution of an Enterococcus faecium isolate from healthy human stool in the absence and presence of a single infecting Myoviridae bacteriophage, EfV12-phi1. Four replicates of E. faecium and phage were grown with twice daily serial transfers for 8 days. Genome sequencing revealed that E. faecium evolved resis

SUBMITTER: Wandro S 

PROVIDER: S-EPMC6365445 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature