The Ferredoxin-Like Protein FerR Regulates PrbP Activity in Liberibacter asiaticus.

ABSTRACT: In Liberibacter asiaticus, PrbP is an important transcriptional accessory protein that regulates gene expression through interactions with the RNA polymerase β-subunit and a specific sequence on the promoter region. The constitutive expression of prbP observed upon chemical inactivation of PrbP-DNA interactions in vivo indicated that the expression of prbP was not autoregulated at the level of transcription. This observation suggested that a modulatory mechanism via protein-protein interactions may be involved. In silico genome association analysis identified FerR (CLIBASIA_01505), a putative ferredoxin-like protein, as a PrbP-interacting protein. Using a bacterial two-hybrid system and immunoprecipitation assays, interactions between PrbP and FerR were c

SUBMITTER: Pan L 

PROVIDER: S-EPMC6365824 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature