Prostate Stroma Increases the Viability and Maintains the Branching Phenotype of Human Prostate Organoids.

ABSTRACT: The fibromuscular stroma of the prostate regulates normal epithelial differentiation and contributes to carcinogenesis in vivo. We developed and characterized a human 3D prostate organoid co-culture model that incorporates prostate stroma. Primary prostate stromal cells increased organoid formation and directed organoid morphology into a branched acini structure similar to what is observed in vivo. Organoid branching occurred distal to physical contact with stromal cells, demonstrating non-random branching. Stroma-induced phenotypes were similar in all patients examined, yet they maintained inter-patient heterogeneity in the degree of response. Stromal cells expressed growth factors involved in epithelial differentiation, which was not observed in non-prostatic fibroblasts. Organoids deriv

SUBMITTER: Richards Z 

PROVIDER: S-EPMC6365938 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature