Long non-coding RNA LINC00346 promotes pancreatic cancer growth and gemcitabine resistance by sponging miR-188-3p to derepress BRD4 expression.

ABSTRACT:

Background

Long non-coding RNA LINC00346 has been recently suggested as a prognostic marker in pancreatic cancer. However, its biological function in pancreatic cancer has not yet been determined. In this study, we attempted to ascertain the role of LINC00346 in regulating the aggressiveness of pancreatic cancer.

Methods

The effects of overexpression and knockdown of LINC00346 on the proliferation, cell cycle progression, apoptosis, and gemcitabine resistance were investigated. Bioinformatic analysis, luciferase reporter assay, and RNA immunoprecipitation assay were performed to search for potential microRNAs (miRs) that can interact with LINC00346.

Results

Overexpression of LINC00346 significantly enhanced the proliferation, colony formation, and tumorigenesis of pan

SUBMITTER: Shi W 

PROVIDER: S-EPMC6366022 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature