Direct pathway cloning of the sodorifen biosynthetic gene cluster and recombinant generation of its product in E. coli.

ABSTRACT:

Background

Serratia plymuthica WS3236 was selected for whole genome sequencing based on preliminary genetic and chemical screening indicating the presence of multiple natural product pathways. This led to the identification of a putative sodorifen biosynthetic gene cluster (BGC). The natural product sodorifen is a volatile organic compound (VOC) with an unusual polymethylated hydrocarbon bicyclic structure (C₁₆H₂₆) produced by selected strains of S. plymuthica. The BGC encoding sodorifen consists of four genes, two of which (sodA, sodB) are homologs of genes encoding enzymes of the non-mevalonate pathway and are thought to enhance the amounts of available farnesyl pyrophosphate (FPP), the precursor of sodorifen. Proceeding from FPP, only two enzymes are necess

SUBMITTER: Duell ER 

PROVIDER: S-EPMC6366047 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature