Rheumatoid arthritis reprograms circadian output pathways.

ABSTRACT:

Objective

We applied systems biology approaches to investigate circadian rhythmicity in rheumatoid arthritis (RA).

Methods

We recruited adults (age 16-80 years old) with a clinical diagnosis of RA (active disease [DAS28 > 3.2]). Sleep profiles were determined before inpatient measurements of saliva, serum, and peripheral blood mononuclear leukocytes (PBML). Transcriptome and proteome analyses were carried out by RNA-SEQ and LC-MS/MS. Serum samples were analysed by targeted lipidomics, along with serum from mouse collagen induced-arthritis (CIA). Bioinformatic analysis identified RA-specific gene networks and rhythmic processes differing between healthy and RA.

Results

RA caused greater time-of-day variation in PBML gene expression, and ex vivo stimulation identified a

SUBMITTER: Poolman TM 

PROVIDER: S-EPMC6366099 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature