An AGT-based protein-tag system for the labelling and surface immobilization of enzymes on E. coli outer membrane.

ABSTRACT: The use of natural systems, such as outer membrane protein A (OmpA), phosphoporin E (PhoE), ice nucleation protein (INP), etc., has been proved very useful for the surface exposure of proteins on the outer membrane of Gram-negative bacteria. These strategies have the clear advantage of unifying in a one-step the production, the purification and the in vivo immobilisation of proteins/biocatalysts onto a specific biological support. Here, we introduce the novel Anchoring-and-Self-Labelling-protein-tag (ASL^tag), which allows the in vivo immobilisation of enzymes on E. coli surface and the labelling of the neosynthesised proteins with the engineered alkylguanine-DNA-alkyl-transferase (H⁵) from Sulfolobus solfataricus. Our results demonstrated that this tag enhanced the ov

SUBMITTER: Merlo R 

PROVIDER: S-EPMC6366409 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature