Chronology of critical events in neonatal rat ventricular myocytes occurring during reperfusion after simulated ischemia.

ABSTRACT: While an ischemic insult poses a lethal danger to myocardial cells, a significant proportion of cardiac myocytes remain viable throughout the ischemic episode and die, paradoxically, only after the blood flow is reinstated. Despite decades of research, the actual chronology of critical events leading to cardiomyocyte death during the reperfusion phase remains poorly understood. Arguably, identification of the pivotal event in this setting is necessary to design effective strategies aimed at salvaging the myocardium after an ischemic attack. Here we used neonatal rat ventricular myocytes (NRVMs) subjected to 20-30 min of simulated ischemia followed by 1 hour of "reperfusion". Using different combinations of spectrally-compatible fluorescent indicators, we analyzed the relative timing of the

SUBMITTER: Sciuto KJ 

PROVIDER: S-EPMC6366697 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature