Highly Selective, Red Emitting BODIPY-Based Fluorescent Indicators for Intracellular Mg²⁺ Imaging.

ABSTRACT: Most fluorescent indicators for Mg²⁺ suffer from poor selectivity against other divalent cations, especially Ca²⁺, thus do not provide reliable information on cellular Mg²⁺ concentrations in processes in which such metals are involved. We report a new set of highly selective fluorescent indicators based on alkoxystyryl-functionalized BODIPY fluorophores decorated with a 4-oxo-4H-quinolizine-3-carboxylic acid metal binding moiety. The new sensors, MagQ1 and MagQ2, display absorption and emission maxima above 600 nm, with a 29-fold fluorescence enhancement and good quantum yields (Φ > 0.3) upon coordination of Mg²⁺ in aqueous buffer. Fluorescence response to Mg²⁺ is not affected by the presence of competing divalent

SUBMITTER: Lin Q 

PROVIDER: S-EPMC6366848 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature