Super-Mendelian inheritance mediated by CRISPR-Cas9 in the female mouse germline.

ABSTRACT: A gene drive biases the transmission of one of the two copies of a gene such that it is inherited more frequently than by random segregation. Highly efficient gene drive systems have recently been developed in insects, which leverage the sequence-targeted DNA cleavage activity of CRISPR-Cas9 and endogenous homology-directed repair mechanisms to convert heterozygous genotypes to homozygosity^1-4. If implemented in laboratory rodents, similar systems would enable the rapid assembly of currently impractical genotypes that involve multiple homozygous genes (for example, to model multigenic human diseases). To our knowledge, however, such a system has not yet been demonstrated in mammals. Here we use an active genetic element that encodes a guide RNA, which is embedded in the mouse ty

SUBMITTER: Grunwald HA 

PROVIDER: S-EPMC6367021 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature