Multifunctional Paper-Based Analytical Device for In Situ Cultivation and Screening of Escherichia coli Infections.

ABSTRACT: Point-of-care testing (POCT) for uropathogen detection and chemical screening has great benefits for the diagnosis of urinary tract infections (UTIs). The goal of this study was to develop a portable and inexpensive paper-based analytical device (PAD) for cultivating bacteria in situ and rapidly testing for nitrite on the same device. The PAD was fabricated using a wax printing technique to create a pattern on Whatman No. 1 filter paper, which was then combined with a cotton sheet to support bacterial growth. Nitrite detection was based on the principle of the Griess reaction, and a linear detection range of 0-1.6 mg/dL (R2 = 0.989) was obtained. Scanning electron microscopy (SEM) analysis demonstrated that the bacteria were able to grow and formed a cluster on the cellulose fibres within

SUBMITTER: Noiphung J 

PROVIDER: S-EPMC6367442 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature