Non-invasive visualisation and identification of fluorescent Leishmania tarentolae in infected sand flies.

ABSTRACT: Background: The leishmaniases are neglected diseases that affect some of the most vulnerable populations in the tropical and sub-tropical world. The parasites are transmitted by sand flies and novel strategies to control this neglected vector-borne disease are needed. Blocking transmission by targeting the parasite inside the phlebotomine vector offers potential in this regard. Some experimental approaches can be best performed by longitudinal study of parasites within flies, for which non-destructive methods to identify infected flies and to follow parasite population changes are required. Methods: Lutzomyia longipalpis were reared under standard insectary conditions at the Wellcome Centre for Molecular Parasitology. Flies were artificially infected with L. tarento

SUBMITTER: Diaz-Albiter HM 

PROVIDER: S-EPMC6367660 | biostudies-literature | 2018

REPOSITORIES: biostudies-literature