Metastasis Suppressor NME1 Directly Activates Transcription of the ALDOC Gene in Melanoma Cells.

ABSTRACT:

Background/aim

NME/NM23 nucleoside diphosphate kinase 1 (NME1) is a metastasis suppressor gene, exhibiting reduced expression in metastatic cancers and the ability to suppress metastatic activity of cancer cells. We previously identified NME1-regulated genes with prognostic value in human melanoma. This study was conducted in melanoma cell lines aiming to elucidate the mechanism through which NME regulates one of these genes, aldolase C (ALDOC).

Materials and methods

ALDOC mRNA and protein expression was measured using qRT-PCR and immunoblot analyses. Promoter-luciferase constructs and chromatin immunoprecipitation were employed to measure the impact of NME1 on ALDOC transcription.

Results

NME1 enhanced ALDOC transcription, evidenced by increased expression of ALDOC p

SUBMITTER: Pamidimukkala NV 

PROVIDER: S-EPMC6367674 | biostudies-literature | 2018 Nov

REPOSITORIES: biostudies-literature