Transcriptional Programming of Normal and Inflamed Human Epidermis at Single-Cell Resolution.

ABSTRACT: Perturbations in the transcriptional programs specifying epidermal differentiation cause diverse skin pathologies ranging from impaired barrier function to inflammatory skin disease. However, the global scope and organization of this complex cellular program remain undefined. Here we report single-cell RNA sequencing profiles of 92,889 human epidermal cells from 9 normal and 3 inflamed skin samples. Transcriptomics-derived keratinocyte subpopulations reflect classic epidermal strata but also sharply compartmentalize epithelial functions such as cell-cell communication, inflammation, and WNT pathway modulation. In keratinocytes, ∼12% of assessed transcript expression varies in coordinate patterns, revealing undescribed gene expression programs governing epidermal homeostasis. We also identi

SUBMITTER: Cheng JB 

PROVIDER: S-EPMC6367716 | biostudies-literature | 2018 Oct

REPOSITORIES: biostudies-literature