Host mRNA decay proteins influence HIV-1 replication and viral gene expression in primary monocyte-derived macrophages.

ABSTRACT: BACKGROUND:Mammalian cells harbour RNA quality control and degradative machineries such as nonsense-mediated mRNA decay that target cellular mRNAs for clearance from the cell to avoid aberrant gene expression. The role of the host mRNA decay pathways in macrophages in the context of human immunodeficiency virus type 1 (HIV-1) infection is yet to be elucidated. Macrophages are directly infected by HIV-1, mediate the dissemination of the virus and contribute to the chronic activation of the inflammatory response observed in infected individuals. Therefore, we characterized the effects of four host mRNA decay proteins, i.e., UPF1, UPF2, SMG6 and Staufen1, on viral replication in HIV-1-infected primary monocyte-derived macrophages (MDMs). RESULTS:Steady-state expression levels of these host mR

SUBMITTER: Rao S 

PROVIDER: S-EPMC6367771 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature