Tailoring the properties of (catalytically)-active inclusion bodies.

ABSTRACT:

Background

Immobilization is an appropriate tool to ease the handling and recycling of enzymes in biocatalytic processes and to increase their stability. Most of the established immobilization methods require case-to-case optimization, which is laborious and time-consuming. Often, (chromatographic) enzyme purification is required and stable immobilization usually includes additional cross-linking or adsorption steps. We have previously shown in a few case studies that the molecular biological fusion of an aggregation-inducing tag to a target protein induces the intracellular formation of protein aggregates, so called inclusion bodies (IBs), which to a certain degree retain their (catalytic) function. This enables the combination of protein production and immobilization in one step.

SUBMITTER: Jager VD 

PROVIDER: S-EPMC6367779 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature