Efficacy of a recombinant single-chain fragment variable region, VasSF, as a new drug for vasculitis.

ABSTRACT:

Background

Anti-neutrophil cytoplasmic autoantibodies (ANCA) associated vasculitis is a pauci-immune disease with the inflammation of the small blood vessels. The efficacies of antibody drugs for induction therapies of vasculitis vary among cases. Here, we developed a novel clone of a single chain Fv region (ScFv) with vasculitis-specific therapeutic potential.

Materials and methods

The clone, termed VasSF, was selected from our Escherichia coli expression library of recombinant human ScFv based on the therapeutic efficacy in an SCG/Kj mouse model of MPO-ANCA-associated vasculitis (MAAV), such as improvement of the urinary score and decreased crescent formation in glomeruli, granulomatous in lung, MPO-ANCA biomarkers, the anti-moesin antibody, and some cytokine levels

SUBMITTER: Kameoka Y 

PROVIDER: S-EPMC6368128 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature