Longdaysin inhibits Wnt/β-catenin signaling and exhibits antitumor activity against breast cancer.

ABSTRACT:

Background

CK1 is involved in regulating Wnt/β-catenin signaling and represents a promising target for the treatment of breast cancer. A purine derivative longdaysin has recently been identified as a novel modulator of cellular circadian rhythms through targeting the protein kinases CK1δ, CK1α, and ERK2. However, the antitumor activity of longdaysin and its underlying mechanisms remain unclear.

Methods

The inhibitory effect of longdaysin on Wnt/β-catenin signaling was investigated using the SuperTOPFlash reporter system. The levels of phosphorylated LRP6, total LRP6, DVL2, active β-catenin, and total β-catenin were examined by Western blot. The expression of Wnt target genes was determined using real-time PCR. The ability of colony formation of breast cancer cells was measur

SUBMITTER: Xiong Y 

PROVIDER: S-EPMC6368421 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature