Importin α Partitioning to the Plasma Membrane Regulates Intracellular Scaling.

ABSTRACT: Early embryogenesis is accompanied by reductive cell divisions requiring that subcellular structures adapt to a range of cell sizes. The interphase nucleus and mitotic spindle scale with cell size through both physical and biochemical mechanisms, but control systems that coordinately scale intracellular structures are unknown. We show that the nuclear transport receptor importin α is modified by palmitoylation, which targets it to the plasma membrane and modulates its binding to nuclear localization signal (NLS)-containing proteins that regulate nuclear and spindle size in Xenopus egg extracts. Reconstitution of importin α targeting to the outer boundary of extract droplets mimicking cell-like compartments recapitulated scaling relationships observed during embryogenesis, which were altere

SUBMITTER: Brownlee C 

PROVIDER: S-EPMC6368448 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature