Condensin-Dependent Chromatin Compaction Represses Transcription Globally during Quiescence.

ABSTRACT: Quiescence is a stress-resistant state in which cells reversibly exit the cell cycle and suspend most processes. Quiescence is essential for stem cell maintenance, and its misregulation is implicated in tumor formation. One of the hallmarks of quiescent cells is highly condensed chromatin. Because condensed chromatin often correlates with transcriptional silencing, it has been hypothesized that chromatin compaction represses transcription during quiescence. However, the technology to test this model by determining chromatin structure within cells at gene resolution has not previously been available. Here, we use Micro-C XL to map chromatin contacts at single-nucleosome resolution genome-wide in quiescent Saccharomyces cerevisiae cells. We describe chromatin domains on the order of 10-60 ki

SUBMITTER: Swygert SG 

PROVIDER: S-EPMC6368455 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature