Development of extracellular matrix supported 3D culture of renal cancer cells and renal cancer stem cells.

ABSTRACT: Novel experimental conditions of cancer cell line culture have evolved throughout the recent years, with significantly growing interest in xeno-free, serum-free and three-dimensional culture variants. The choice of proper culture media may enable to mimic tumor microenvironment and promotion of cancer stem cells proliferation. To assess whether stem-like phenotype inducing media may be applied in renal cancer stem cell research, we performed a widespread screening of 13 cell culture media dedicated for mesenchymal cells, stem cells as well as mesenchymal stem cells. We have also screened extracellular matrix compounds and selected optimal RCC 3D-ECM supported culture model. Our results revealed that 786-O as well as HKCSCs cell line cultures in xeno-free media (NutriStem/StemXvivo) and lam

SUBMITTER: Maliszewska-Olejniczak K 

PROVIDER: S-EPMC6368519 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature