The binding of Borealin to microtubules underlies a tension independent kinetochore-microtubule error correction pathway.

ABSTRACT: Proper chromosome segregation depends upon kinetochore phosphorylation by the Chromosome Passenger Complex (CPC). Current models suggest the activity of the CPC decreases in response to the inter-kinetochore stretch that accompanies the formation of bi-oriented microtubule attachments, however little is known about tension-independent CPC phosphoregulation. Microtubule bundles initially lie in close proximity to inner centromeres and become depleted by metaphase. Here we find these microtubules control kinetochore phosphorylation by the CPC in a tension independent manner via a microtubule-binding site on the Borealin subunit. Disruption of Borealin-microtubule interactions generates reduced phosphorylation of prometaphase kinetochores, improper kinetochore-microtubule attachments and weak

SUBMITTER: Trivedi P 

PROVIDER: S-EPMC6368601 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature