Factorial design-assisted supercritical carbon-dioxide extraction of cytotoxic active principles from Carica papaya leaf juice.

ABSTRACT: The aims of this study are to investigate the selective cytotoxic activity of supercritical carbon dioxide (scCO₂)-extracted freeze-dried leaf juice (FDLJ) of Carica papaya on squamous cell carcinoma (SCC25) cells, and to delineate the best small scale extraction parameters allowing maximal extract activity. Using scCO₂ as a solvent, six operating parameters were studied and the supercritical fluid extraction (SFE) process investigated using a factorial design 2^6-2. The processing values promoting cytotoxic activity towards SCC-25 are: high pressure (250 bar), low temperature (35 °C), extended processing time (180 minutes), as well as a large amount of starting material (5 g). The factorial experimental design successfully identified the key parameters cont

SUBMITTER: Khaw KY 

PROVIDER: S-EPMC6368614 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature