FUS/circ_002136/miR-138-5p/SOX13 feedback loop regulates angiogenesis in Glioma.

ABSTRACT:

Background

Angiogenesis plays a critical role in the progression of glioma. Previous studies have indicated that RNA-binding proteins (RBPs) interact with RNAs and participate in the regulation of the malignant behaviors of tumors. As a type of endogenous non-coding RNAs, circular RNAs (circRNAs) are abnormally expressed in various cancers and are involved in diverse tumorigeneses including angiogenesis.

Methods

The expression levels of FUS, circ_002136, miR-138-5p, SOX13, and SPON2 were determined using quantitative real-time PCR (qRT-PCR) and western blot. Transient cell transfection was performed using the Lipofectamine 3000 reagent. The RNA-binding protein immunoprecipitation (RNA-IP) and the RNA pull-down assays were used to detect the interaction between FUS and circ_0

SUBMITTER: He Z 

PROVIDER: S-EPMC6368736 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature