Amblyomma sculptum Salivary PGE₂ Modulates the Dendritic Cell-Rickettsia rickettsii Interactions in vitro and in vivo.

ABSTRACT: Amblyomma sculptum is an important vector of Rickettsia rickettsii, causative agent of Rocky Mountain spotted fever and the most lethal tick-borne pathogen affecting humans. To feed on the vertebrate host's blood, A. sculptum secretes a salivary mixture, which may interact with skin resident dendritic cells (DCs) and modulate their function. The present work was aimed at depicting the A. sculptum saliva-host DC network and the biochemical nature of the immunomodulatory component(s) involved in this interface. A. sculptum saliva inhibits the production of inflammatory cytokines by murine DCs stimulated with LPS. The fractionation of the low molecular weight salivary content by reversed-phase chromatography revealed active fractions eluting from 49 to 55% o

SUBMITTER: Esteves E 

PROVIDER: S-EPMC6369204 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature