Microfluidic reactors for advancing the MS analysis of fast biological responses.

ABSTRACT: The response of cells to physical or chemical stimuli is complex, unfolding on time-scales from seconds to days, with or without de novo protein synthesis, and involving signaling processes that are transient or sustained. By combining the technology of microfluidics that supports fast and precise execution of a variety of cell handling operations, with that of mass spectrometry detection that facilitates an accurate and complex characterization of the protein complement of cells, in this work, we developed a platform that supports (near) real-time sampling and proteome-level capturing of cellular responses to a perturbation such as treatment with mitogens. The geometric design of the chip supports three critical features: (a) capture of a sufficient number of cells to meet the detection l

SUBMITTER: Lazar IM 

PROVIDER: S-EPMC6369226 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature