Cytochrome c autocatalyzed carbonylation in the presence of hydrogen peroxide and cardiolipins.

ABSTRACT: Cytochrome c (cyt c) is a small hemoprotein involved in electron shuttling in the mitochondrial respiratory chain and is now also recognized as an important mediator of apoptotic cell death. Its role in inducing programmed cell death is closely associated with the formation of a complex with the mitochondrion-specific phospholipid cardiolipin (CL), leading to a gain of peroxidase activity. However, the molecular mechanisms behind this gain and eventual cyt c autoinactivation via its release from mitochondrial membranes remain largely unknown. Here, we examined the kinetics of the H₂O₂-mediated peroxidase activity of cyt c both in the presence and absence of tetraoleoyl cardiolipin (TOCL)- and tetralinoleoyl cardiolipin (TLCL)-containing lip

SUBMITTER: Barayeu U 

PROVIDER: S-EPMC6369287 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature