Detection and clearance of a mosquito densovirus contaminant from laboratory stocks of Zika virus.

ABSTRACT:

Background

The Zika virus (ZIKV) epidemics that affected South America in 2016 raised several research questions and prompted an increase in studies in the field. The transient and low viraemia observed in the course of ZIKV infection is a challenge for viral isolation from patient serum, which leads to many laboratories around the world sharing viral strains for their studies. C6/36 cells derived from Aedes albopictus larvae are commonly used for arbovirus isolation from clinical samples and for the preparation of viral stocks.

Objectives

Here, we report the contamination of two widely used ZIKV strains by Brevidensovirus, here designated as mosquito densovirus (MDV).

Methods

Molecular and immunological techniques were used to analyse the MDV contamination of ZIKV st

SUBMITTER: Cataneo AHD 

PROVIDER: S-EPMC6369503 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature