Probe Sensitivity to Cortical versus Intracellular Cytoskeletal Network Stiffness.

ABSTRACT: In development, wound healing, and pathology, cell biomechanical properties are increasingly recognized as being of central importance. To measure these properties, experimental probes of various types have been developed, but how each probe reflects the properties of heterogeneous cell regions has remained obscure. To better understand differences attributable to the probe technology, as well as to define the relative sensitivity of each probe to different cellular structures, here we took a comprehensive approach. We studied two cell types-Schlemm's canal endothelial cells and mouse embryonic fibroblasts (MEFs)-using four different probe technologies: 1) atomic force microscopy (AFM) with sharp tip, 2) AFM with round tip, 3) optical magnetic twisting cytometry (OMTC), and 4) traction mic

SUBMITTER: Vahabikashi A 

PROVIDER: S-EPMC6369565 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature