Adapted MS/MS^ALL Shotgun Lipidomics Approach for Analysis of Cardiolipin Molecular Species.

ABSTRACT: Cardiolipin (Ptd₂ Gro) is a complex, doubly charged phospholipid located in the inner mitochondrial membrane where it plays an essential role in regulating bioenergetics. Abnormalities in Ptd₂ Gro content or composition have been associated with mitochondrial dysfunction in a variety of disease states. Here, we report the development of an adapted high-resolution data-independent acquisition (DIA) MS/MS^ALL shotgun lipidomic method to enhance the accuracy and reproducibility of Ptd₂ Gro molecular species quantitation from biological samples. Utilizing the doubly charged molecular ions and the isotopic pattern with negative mode electrospray ionization mass spectrometry (ESI-MS) using an adapted MS/MS^ALL approach, we profiled more than 1

SUBMITTER: Gao F 

PROVIDER: S-EPMC6369710 | biostudies-literature | 2018 Jan

REPOSITORIES: biostudies-literature