More than 18,000 effectors in the <i>Legionella</i> genus genome provide multiple, independent combinations for replication in human cells.

ABSTRACT: The genus Legionella comprises 65 species, among which Legionella pneumophila is a human pathogen causing severe pneumonia. To understand the evolution of an environmental to an accidental human pathogen, we have functionally analyzed 80 Legionella genomes spanning 58 species. Uniquely, an immense repository of 18,000 secreted proteins encoding 137 different eukaryotic-like domains and over 200 eukaryotic-like proteins is paired with a highly conserved type IV secretion system (T4SS). Specifically, we show that eukaryotic Rho- and Rab-GTPase domains are found nearly exclusively in eukaryotes and Legionella Translocation assays for selected Rab-GTPase proteins revealed that they are indeed T4SS secreted substrates. Furthermore, F-box, U-box, and SET domains were

SUBMITTER: Gomez-Valero L 

PROVIDER: S-EPMC6369783 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature