Multiomics resolution of molecular events during a day in the life of Chlamydomonas.

ABSTRACT: The unicellular green alga Chlamydomonas reinhardtii displays metabolic flexibility in response to a changing environment. We analyzed expression patterns of its three genomes in cells grown under light-dark cycles. Nearly 85% of transcribed genes show differential expression, with different sets of transcripts being up-regulated over the course of the day to coordinate cellular growth before undergoing cell division. Parallel measurements of select metabolites and pigments, physiological parameters, and a subset of proteins allow us to infer metabolic events and to evaluate the impact of the transcriptome on the proteome. Among the findings are the observations that Chlamydomonas exhibits lower respiratory activity at night compared with the day; multiple fermentation pathwa

SUBMITTER: Strenkert D 

PROVIDER: S-EPMC6369806 | biostudies-literature | 2019 Feb

REPOSITORIES: biostudies-literature