Membrane potential (V_mem) measurements during mesenchymal stem cell (MSC) proliferation and osteogenic differentiation.

ABSTRACT:

Background

Electrochemical signals play an important role in cell communication and behavior. Electrically charged ions transported across cell membranes maintain an electrochemical imbalance that gives rise to bioelectric signaling, called membrane potential or V_mem. V_mem plays a key role in numerous inter- and intracellular functions that regulate cell behaviors like proliferation, differentiation and migration, all playing a critical role in embryonic development, healing, and regeneration.

Methods

With the goal of analyzing the changes in V_mem during cell proliferation and differentiation, here we used direct current electrical stimulation (EStim) to promote cell proliferation and differentiation and simultaneously tracked the correspondi

SUBMITTER: Bhavsar MB 

PROVIDER: S-EPMC6369823 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature