Improved Loading of Plasma-Derived Extracellular Vesicles to Encapsulate Antitumor miRNAs.

ABSTRACT: Extracellular vesicles (EVs) carry various molecules involved in intercellular communication and have raised great interest as drug delivery systems. Several engineering methods have been investigated for vesicle loading. Here, we studied the electroporation of EVs isolated from plasma to load antitumor microRNAs (miRNAs). First, we optimized the transfection protocol using miRNA cel-39 by evaluating different parameters (voltage and pulse) for their effect on vesicle morphology, loading capacity, and miRNA transfer to target cells. When compared with direct incubation of EVs with miRNA, mild electroporation allowed more efficient loading and better protection of miRNA from RNase degradation. Moreover, electroporation preserved the naive vesicle cargo, including RNAs and proteins, and thei

SUBMITTER: Pomatto MAC 

PROVIDER: S-EPMC6370572 | biostudies-literature | 2019 Jun

REPOSITORIES: biostudies-literature